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Hospital, Medical Center,Self High AccuracyAnti-TP ELISA Test Kit

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Hospital, Medical Center,Self High AccuracyAnti-TP ELISA Test Kit

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Brand Name :BIOVANTION
Model Number :TY0031
Certification :ISO 13485
Place of Origin :CHINA
MOQ :1000 Box
Price :discussible
Payment Terms :L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability :100000 Box/carton
Delivery Time :2-7 days
Packaging Details :carton/box
Product name :NS1-IgM/IgG
Packaging Specifications :50 tests/box
Country Of Origin :China,BeiJing
Detection Limit :24 months
Storage :2-8℃
Specimen :serum, plasma, whole blood,fingertip blood
Instrument classification :Class I
Product Type :Repid Test
Accuracy :99.9%
Application :Hospital, Medical Center,self
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Hospital, Medical Center,Self High AccuracyAnti-TP ELISA Test Kit

INTENDED USE

The Anti-TP ELISA is a qualitative enzyme immunoassay for the in vitro detection of Treponema Pallidum (TP) infection, based on the detection of total antibody to Treponema Pallidum in human serum or plasma. It is intended for screening of blood donors and diagnosing patients related to infection with TP.

Hospital, Medical Center,Self High AccuracyAnti-TP ELISA Test Kit

Product Detail Description
Packaging Specifications 50 tests/box
Country Of Origin China,BeiJing
Detection Limit 24 months
Storage 4-30℃
Specimen serum, plasma, whole blood,fingertip blood
Instrument classification Class I
Product Type Repid Test
Accuracy 99.9%
Application Hospital, Medical Center,self

ASSAY PROCEDURE

  • Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×)with 19 volumes of distilled water, mix well.
  • Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control. After dispensing 50μL of Sample Diluent , dispense 50μL of sample or negative control or positive control to the respective wells. Gently vibrating the plate.
  • Incubate: Cover the Microplate with plate cover and incubate the Coated Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 60 minutes.
  • Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
  • Add Conjugate: Add 100μL of conjugate to each well (except for the blank well).
  • Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
  • Wash the Plate: Repeat the wash procedure as in step 4.
  • Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well. Cover and incubate at 37℃ for 30 minutes.
  • Stop reaction: Add 50μL Stop Solution to each well, mix well.
  • Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.

PRECAUTIONS

  • Allow all kit components to reach room temperature before use.
  • Follow the direction insert to control the reaction temperature and time strictly.
  • Do not mix components of different lot numbers to use.
  • The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.

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