Biovantion Inc.
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Research test for the quantitative determination of SAA Kit in human serum on automate chemistry analyzers
SAA (Serum Amyloid A) is is an acute-phase protein. During
inflammation, infectious and non-infectious diseases, its concentration in the blood can rise sharply within a few hours, and can rise to 1000 times the initial concentration. SAA is
related to HDL, and it can It regulates the metabolism of HDL
during inflammation. A particularly important feature of SAA is
that its degradation products can be deposited in different organs
in the form of amyloid A fibrils. This is a serious complication in
chronic inflammatory diseases; Elevated serum amyloid A is also
seen in atherosclerosis, diabetic nephropathy, acute myocardial
infarction, coronary heart disease, and chronic kidney disease
Components
| Contents | components | Concentration |
|
R1 | PBS Buffer NaCl Surfactant Antiseptic | 0.1 mol/L Titer Titer Titer |
|
R2 | Latex particles coated with SAA antibody PB Buffer NaCl Surfactant Antiseptic | >0.5mg/L
0.1 mol/L Titer Titer Titer |
|
Calibrator /Control | SAA Bovine serum albumin NaCl Surfactant Antiseptic | See Value Sheet Titer Titer Titer Titer |
Reagent preparation:
R1: Ready for use; R2: Ready for use. Wavelength:570nm Temperature:37℃
Direction: Increase
| Blank Tube | Calibration Tube | Sample Tube | |
| Sample | － | － | 2ul |
| Calibrator | － | 2ul | － |
| Deionized water | 2ul | － | － |
| R1 | 200μl | 200μl | 200μl |
| Mix, incubate at 37℃ for 5 minutes. | |||
| R2 | 40μl | 40μl | 40μl |
| Mix, incubate at 37℃ for 5 minutes, zero setting for blank tube, read the absorbance △A calibrator and △A sample | |||
It is recommended to use the SAA Calibrators for calibration. Dissolve the lyophilized SAA calibrator with 1ml distilled water, and dilute to 6 calibrators as follows,
| Dilute | 1 | 2 | 3 | 4 | 5 | 6 |
| CAL (μl) | 200 | 100 | 50 | 50 | 25 | 0 |
| Normal Saline (μl) | 0 | 100 | 150 | 350 | 375 | 200 |
| ratio | 1 | 1/2 | 1/4 | 1/8 | 1/16 | 0 |
Calibration Method:Spline or other non-linear method
It is recommended to use the SAA Quality Control level 1 and level 2 for daily quality control.
Two levels of controls should be assayed at least once a day. Values obtained should fall within a specified range.
Each laboratory should establish its own internal Quality Control scheme and procedures for corrective action if controls do not recover within the acceptable tolerances.
The analyzer automatically calculate the analyte concentration of each sample.