Sheep Interleukin 1 Beta Elisa Kit IL1F2 ELISA RUO Test Kit Il1b Elisa Kit

Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B;IL1F2 ELISA Kit

Sheep Interleukin 1 Beta,IL-1Beta; IL-1B;IL1B ELISA Kit

• For research use only.
• For the quantitative in vitro determination of Sheep Interleukin 1 Beta(IL-1B) concentrations in Sheep serum, plasma, culture media or any biological fluid.
• Expiration date:six months .
• Storage: 2-8℃.
• Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1B Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-1B is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1B and HRP conjugated IL-8 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1B. You can calculate the concentration of IL-1B in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1B were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1B were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Assay range

3pg/ml -200pg/ml

Sensitivity:

0.5pg/ml