Serum 96 Pcs COVID-19 Test Kit 60 Minutes Human HBeAb Elisa Kit

Human HBeAb Elisa Kit 96 Test/Kit

HBeAb ELISA Kit
CatalogNo.: BE104A

Enzyme-linked immunosorbent assay for the detection antibody against HBeAg in serum or plasma

1. SUMMARY AND PRINCIPLE OF THE TEST

The presence of antibody against hepatitis B viral e antigen is used as an indicator for (1) early HBs antigenemia before the peak of viral replication and (2) early convalescence when HBeAg has declined below detectable levels. It is also useful to confirm a seroconversion. The seroconversion from HBeAg positivity to anti-HBe positivity indicates a reduced level of infectious virus because virus replication has decreased.

REAGENTS

2 . Materials provided with the kits:
1.Microtiter Well coated with purified anti-HBe: 96 tests
2.Negative Control: One vial of 0. 5ml anti-HBe Negative Control.
3.Positive Control: One vial of 0. 5ml anti-HBe Positive Control.
4.Enzyme Conjugate: 6 ml containing HRP-conjugated-anti-HBe-HBeAg complex for 96 tests.
5.Wash Buffer Concentrate (20 x): 20 ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
6.Substrate Solution A: 6 ml HRP Substrate for 96 tests.
7.Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
8.Stop Solution: One bottle of 6 ml 2N Sulfuric Acid.

3. ASSAY PROCEDURE

1. Allow all reagents to reach room temperature before use.
2. Check the Wash buffer concentrate for the presence of salt crystals. If crystals have formed in the solution, resolubilize by warming at 37℃ until crystals dissolve. Dilute concentrated wash buffer 1:19 with ddH2O. Use only clean vessels to dilute the buffer.
3. For each test, set two positive and two negative controls. Dispense one drop (50 ul) of Positive Control as well as Negative Control in duplicate into respective wells. Set one black well as background control, and 50ul of serum or plasma samples into respective wells.
4. Add one drop (50 ul) of Enzyme Conjugate to each well. Mix it gently by swirling the microtiter plate on flat bench for 1 min. Do not add Enzyme Conjugate to the blank well.
5. Place the microtiter plate into a humidified box and incubate at 37°C for 30 min.
6. Wash each well 5 times by filling each well with diluted wash buffer, then invert the plate vigorously to get all water out and block the rim of each well on absorbent paper for a few seconds.
7. Add one drop (50 ul) of Substrate Solution A to each well, then add one drop (50 ul) of Substrate Solution B to each well. Mix gently and incubate at 37°C for 15 min.
8. Add one drop (50 ul) of Stop Solution to each well to stop the color reaction. Read O.D. at 450 nm(450 nm/630 nm) with an EIA reader.