Biovantion Inc.
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Hot Sale HIV Ab & Ag ELISA 96 Test/Kit
Catalog No.: BE701A
1. INTENDED USE
2. Materials provided with the kits:
| Items | Description | 96T |
| 1. | Microplate | 96 wells |
| 2. | Negative Control | 1 mL ×1 |
| 3. | Positive Control HIV-1 | 1 mL ×1 |
| 4. | Positive Control HIV-2 | 1 mL ×1 |
| 5. | Positive Control P24 Antigen | 1 mL ×1 |
| 6. | Conjugate 1 | 3 mL ×1 |
| 7. | Conjugate 2 | 12 mL ×1 |
| 8. | Washing Buffer | 40 mL ×1 |
| 9. | Substrate Solution A | 6 mL ×1 |
| 10. | Substrate Solution B | 6 mL ×1 |
| 11. | Stop Solution | 6 mL ×1 |
| 12. | Plate Cover | 3 piececs |
| 13. | Insert | 1 copy |
3. ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Washing Buffer with 19 volumes of distilled water, mix well.
2. Add Conjugate 1 and Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2
wells as Negative Control, 2 wells as Positive Control HIV-1, 2 wells Positive Control HIV-2 and 2 wells
Positive Control P24 Antigen. After dispensing 75μL of sample or Negative Control or Positive Control to
the respective wells, dispense 25μL of Conjugate 1 to each well (except the blank well). Gently vibrating
the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled
water-bath or microplate incubator at 37℃ for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of all wells. Fill the wells with the diluted
washing buffer (10~20 seconds to soak) then aspirate again. Repeat the procedure for 5 times. Make sure
that the rest volume is minimal, by tapping plate onto absorbent paper.
5. Add Conjugate 2: Add 100μL of Conjugate 2 to each well (except the blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, mix well.
Cover and incubate at 37℃ for 30 minutes.
9. Stop reaction: Add 50μL Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.