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Interferon Gamma Release Assay (IGRA) measure a person’s immune reactivity to M.tuberculosis. T
lymphocytes from most persons that have been infected with M.tuberculosis will release IFN-γ when
mixed with special antigens derived from M.tuberculosis. IGRA is whole-blood test that can aid in
diagnosing Mycobacterium tuberculosis infection, including both latent tuberculosis infection (LTBI) and
tuberculosis (TB) disease
ELISA Components
1. Microplate Strips 96 well
2. Human IFN-γ Standard, lyophilised (Blue lid) 2 x vial
3. Diluent 1 x 40mL
4. Biotin Labeled Antibody (Yellow cap) 1 x 6mL
5. Enzyme Conjugate 100X Concentrate (Brown bottle)1 x 130μL
6. Wash Buffer 10X Concentrate 1 x 40mL
7. Chromogen Solution A 1 x 6mL
8. Chromogen Solution B 1 x 6mL
9. Stopping Solution 1 x 6mL